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One-Pot CRISPR Test Spots Deadly Mpox Clade in 40 Minutes
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One-Pot CRISPR Test Spots Deadly Mpox Clade in 40 Minutes
Researchers have developed a one-pot RPA-CRISPR/Cas12a assay that visually detects monkeypox virus clade I within 40 minutes, achieving a 95 percent limit of detection of 27.16 copies per microliter and outperforming qPCR on low-copy simulated clinical samples.
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