Genome Editing that Avoids Immune Detection to Integrate Large DNA Sequences

Genome editing that avoids immune detection to integrate large DNA sequences

A genome-editing approach called INSTALL combines single-stranded DNA molecules that evade immune detection with a short double-stranded region that allows recombinase enzymes to insert a DNA sequence into mammalian genomes. INSTALL enables the insertion of large DNA sequences into the genomes of human and mouse cells without causing toxic immune responses. Single-stranded DNA molecules with a small double-stranded region enable gene insertion w…

Metapipeline-DNA Automates and Standardizes Genome Sequencing Analysis

In a single experiment, scientists can decipher the entire genomes of many patient samples, animal models or cultured cells. To fully realize the potential to study biology at this unprecedented scale, researchers must be equipped to analyze the massive amounts  of data generated by these new methods. Scientists headed by a team at Sanford Burnham Prebys Medical Discovery Institute and the University of California Los Angeles have now reported o…